› 3n1f

X-ray diffraction
1.6Å resolution

Crystal Structure of IhhN bound to CDOFn3

Released:
DOI: 10.2210/pdb3n1f/pdb
PDB entry 3n1f coloured by chain, front view.
PDB entry 3n1f coloured by chain, side view.
PDB entry 3n1f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner.
Kavran JM, Ward MD, Oladosu OO, Mulepati S, Leahy DJ
J Biol Chem 285 24584-90 (2010)
PMID: 20519495

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172046 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Indian hedgehog protein N-product Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 19.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14623 (Residues: 29-193; Coverage: 43%)
Gene name: IHH
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2
Cell adhesion molecule-related/down-regulated by oncogenes Chains: C, D
Molecule details ›
Chains: C, D
Length: 102 amino acids
Theoretical weight: 11.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4KMG0 (Residues: 826-924; Coverage: 8%)
Gene names: CDO, CDON
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2221
Unit cell:
a: 71.758Å b: 98.198Å c: 144.105Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.189
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The Hedgehog signal transduction network.
Robbins et al. (2012)
6 more

3 mentions without citation

All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner.
Kavran et al. (2010)
2 more