› 3n1q

X-ray diffraction
2.89Å resolution

Crystal Structure of DhhN bound to CDOFn3

Released:
DOI: 10.2210/pdb3n1q/pdb
PDB entry 3n1q coloured by chain, front view.
PDB entry 3n1q coloured by chain, side view.
PDB entry 3n1q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner.
Kavran JM, Ward MD, Oladosu OO, Mulepati S, Leahy DJ
J Biol Chem 285 24584-90 (2010)
PMID: 20519495

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128898 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Desert hedgehog protein N-product Chains: A, B, E
Molecule details ›
Chains: A, B, E
Length: 170 amino acids
Theoretical weight: 19.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43323 (Residues: 24-189; Coverage: 44%)
Gene name: DHH
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2
Cell adhesion molecule-related/down-regulated by oncogenes Chains: C, D, F
Molecule details ›
Chains: C, D, F
Length: 102 amino acids
Theoretical weight: 11.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4KMG0 (Residues: 826-924; Coverage: 8%)
Gene names: CDO, CDON
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 45.715Å b: 100.265Å c: 97.26Å
α: 90° β: 98.98° γ: 90°
R-values:
R R work R free
0.24 0.237 0.288
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The Hedgehog signal transduction network.
Robbins et al. (2012)
6 more

2 mentions without citation

All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner.
Kavran et al. (2010)
1 more