› 3n1u

X-ray diffraction
1.8Å resolution

Structure of putative HAD superfamily (subfamily III A) hydrolase from Legionella pneumophila

Released:
DOI: 10.2210/pdb3n1u/pdb
PDB entry 3n1u coloured by chain, front view.
PDB entry 3n1u coloured by chain, side view.
PDB entry 3n1u coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Entry authors: Ramagopal UA, Toro R, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.46 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZX93 (Residues: 4-183; Coverage: 98%)
Gene name: lpg0839
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 80.907Å b: 80.907Å c: 65.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.217
Expression system: Escherichia coli BL21(DE3)

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