› 3n7z

X-ray diffraction
2.75Å resolution

Crystal structure of acetyltransferase from Bacillus anthracis

Released:
DOI: 10.2210/pdb3n7z/pdb
PDB entry 3n7z coloured by chain, front view.
PDB entry 3n7z coloured by chain, side view.
PDB entry 3n7z coloured by chain, top view.
Source organism: Bacillus anthracis str. Sterne
Primary publication:
Biochemical and structural analysis of an Eis family aminoglycoside acetyltransferase from bacillus anthracis.
Green KD, Biswas T, Chang C, Wu R, Chen W, Janes BK, Chalupska D, Gornicki P, Hanna PC, Tsodikov OV, Joachimiak A, Garneau-Tsodikova S
Biochemistry 54 3197-206 (2015)
PMID: 25928210

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyltransferase, GNAT family Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 388 amino acids
Theoretical weight: 46.46 KDa
Source organism: Bacillus anthracis str. Sterne
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 6 x NA
1 modified residue:
Ligand MSE 84 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 79.429Å b: 176.859Å c: 109.973Å
α: 90° β: 105.73° γ: 90°
R-values:
R R work R free
0.19 0.187 0.242
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Comprehensive review of chemical strategies for the preparation of new aminoglycosides and their biological activities.
Thamban Chandrika et al. (2018)
1 more

4 mentions without citation

Strategies to overcome the action of aminoglycoside-modifying enzymes for treating resistant bacterial infections.
Labby et al. (2013)
3 more