› 3n81

X-ray diffraction
1.7Å resolution

T244A mutant of Human mitochondrial aldehyde dehydrogenase, apo form

Released:
DOI: 10.2210/pdb3n81/pdb
PDB entry 3n81 coloured by chain, front view.
PDB entry 3n81 coloured by chain, side view.
PDB entry 3n81 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Gonzalez-Segura L, Hurley TD

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 500 amino acids
Theoretical weight: 54.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05091 (Residues: 18-517; Coverage: 97%)
Gene names: ALDH2, ALDM
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 8 x NA
Ligand GAI 21 x GAI
Ligand EDO 27 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 141.75Å b: 152.194Å c: 177.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.217
Expression system: Escherichia coli BL21(DE3)

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