› 3ncy

X-ray diffraction
3.2Å resolution

X-ray crystal structure of an arginine agmatine antiporter (AdiC) in complex with a Fab fragment

Released:
DOI: 10.2210/pdb3ncy/pdb
PDB entry 3ncy coloured by chain, front view.
PDB entry 3ncy coloured by chain, side view.
PDB entry 3ncy coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a prokaryotic virtual proton pump at 3.2 A resolution.
Fang Y, Jayaram H, Shane T, Kolmakova-Partensky L, Wu F, Williams C, Xiong Y, Miller C
Nature 460 1040-3 (2009)
PMID: 19578361

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211410 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Arginine/agmatine antiporter Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 445 amino acids
Theoretical weight: 46.96 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60066 (Residues: 1-445; Coverage: 100%)
Gene names: STM4294, adiC, aniC
Sequence domains: Amino acid permease
Fab Heavy chain Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 219 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Fab Light chain Chains: S, W
Molecule details ›
Chains: S, W
Length: 211 amino acids
Theoretical weight: 23.19 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B, ALS BEAMLINE 8.2.2, NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 79.661Å b: 104.149Å c: 154.025Å
α: 81.96° β: 75.93° γ: 73.73°
R-values:
R R work R free
0.283 0.282 0.312
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

37 review citations

Biology of human sodium glucose transporters.
Wright et al. (2011)
36 more

18 mentions without citation

Glycines: role in α-helical membrane protein structures and a potential indicator of native conformation.
Dong et al. (2012)
17 more