› 3nfl

X-ray diffraction
1.91Å resolution

Crystal structure of the PTPN4 PDZ domain complexed with the C-terminus of the GluN2A NMDA receptor subunit

Released:
DOI: 10.2210/pdb3nfl/pdb
PDB entry 3nfl coloured by chain, front view.
PDB entry 3nfl coloured by chain, side view.
PDB entry 3nfl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Peptides targeting the PDZ domain of PTPN4 are efficient inducers of glioblastoma cell death.
Babault N, Cordier F, Lafage M, Cockburn J, Haouz A, Prehaud C, Rey FA, Delepierre M, Buc H, Lafon M, Wolff N
Structure 19 1518-24 (2011)
PMID: 22000519

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-151344 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 107 amino acids
Theoretical weight: 11.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29074 (Residues: 499-604; Coverage: 11%)
Gene name: PTPN4
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
Glutamate receptor ionotropic, NMDA 2A Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 16 amino acids
Theoretical weight: 1.91 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q12879 (Residues: 1449-1464; Coverage: 1%)
Gene names: GRIN2A, NMDAR2A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 51.582Å b: 51.679Å c: 190.902Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.217 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Rabies.
Fooks et al. (2017)
6 more

1 mention without citation

Structural and functional characterization of the PDZ domain of the human phosphatase PTPN3 and its interaction with the human papillomavirus E6 oncoprotein.
Genera et al. (2019)