› 3nfp

X-ray diffraction
2.86Å resolution

Crystal structure of the Fab fragment of therapeutic antibody daclizumab in complex with IL-2Ra (CD25) ectodomain

Released:
DOI: 10.2210/pdb3nfp/pdb
PDB entry 3nfp coloured by chain, front view.
PDB entry 3nfp coloured by chain, side view.
PDB entry 3nfp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of immunosuppression by the therapeutic antibody daclizumab.
Yang H, Wang J, Du J, Zhong C, Zhang D, Guo H, Guo Y, Ding J
Cell Res 20 1361-71 (2010)
PMID: 20820193

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233899 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heavy chain of Fab fragment of daclizumab Chains: A, H
Molecule details ›
Chains: A, H
Length: 216 amino acids
Theoretical weight: 23.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Light chain of Fab fragment of daclizumab Chains: B, L
Molecule details ›
Chains: B, L
Length: 212 amino acids
Theoretical weight: 23.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Interleukin-2 receptor subunit alpha Chains: I, K
Molecule details ›
Chains: I, K
Length: 223 amino acids
Theoretical weight: 25.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01589 (Residues: 22-238; Coverage: 87%)
Gene name: IL2RA
Sequence domains: Sushi repeat (SCR repeat)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 102.167Å b: 114.973Å c: 247.555Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.253 0.301
Expression systems:
  • Not provided
  • Spodoptera frugiperda

Quick links

Citations

8 review citations

Impact of Immune-Modulatory Drugs on Regulatory T Cell.
Furukawa et al. (2016)
7 more

8 mentions without citation

OptMAVEn--a new framework for the de novo design of antibody variable region models targeting specific antigen epitopes.
Li et al. (2014)
7 more