› 3nkd

X-ray diffraction
1.95Å resolution

Structure of CRISP-associated protein Cas1 from Escherichia coli str. K-12

Released:
DOI: 10.2210/pdb3nkd/pdb
PDB entry 3nkd coloured by chain, front view.
PDB entry 3nkd coloured by chain, side view.
PDB entry 3nkd coloured by chain, top view.
Source organism: Escherichia coli DH1
Primary publication:
A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair.
Babu M, Beloglazova N, Flick R, Graham C, Skarina T, Nocek B, Gagarinova A, Pogoutse O, Brown G, Binkowski A, Phanse S, Joachimiak A, Koonin EV, Savchenko A, Emili A, Greenblatt J, Edwards AM, Yakunin AF
Mol Microbiol 79 484-502 (2011)
PMID: 21219465

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 33.24 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46896 (Residues: 1-305; Coverage: 100%)
Gene names: JW2725, b2755, cas1, ygbT
Sequence domains: CRISPR associated protein Cas1
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 78.674Å b: 79.3Å c: 101.562Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

56 review citations

Evolution and classification of the CRISPR-Cas systems.
Makarova et al. (2011)
55 more

7 mentions without citation

Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity.
Nuñez et al. (2014)
6 more