3nke

X-ray diffraction
1.4Å resolution

High resolution structure of the C-terminal domain CRISP-associated protein Cas1 from Escherichia coli str. K-12

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo trimer (preferred)
PDBe Complex ID:
PDB-CPX-175158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 200 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46896 (Residues: 92-291; Coverage: 66%)
Gene names: JW2725, b2755, cas1, ygbT
Sequence domains: CRISPR associated protein Cas1
Structure domains: CRISPR-associated endonuclease Cas1, C-terminal domain

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 53.709Å b: 90.047Å c: 121.876Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.201
Expression system: Escherichia coli BL21(DE3)