› 3nkv

X-ray diffraction
1.7Å resolution

Crystal structure of Rab1b covalently modified with AMP at Y77

Released:
DOI: 10.2210/pdb3nkv/pdb
PDB entry 3nkv coloured by chain, front view.
PDB entry 3nkv coloured by chain, side view.
PDB entry 3nkv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Legionella effector protein DrrA AMPylates the membrane traffic regulator Rab1b.
Müller MP, Peters H, Blümer J, Blankenfeldt W, Goody RS, Itzen A
Science 329 946-9 (2010)
PMID: 20651120

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190497 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H0U4 (Residues: 3-174; Coverage: 86%)
Gene name: RAB1B
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
Ligand BA 2 x BA
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 34.97Å b: 100.589Å c: 45.071Å
α: 90° β: 102.43° γ: 90°
R-values:
R R work R free
0.152 0.15 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

68 review citations

Role of Rab GTPases in membrane traffic and cell physiology.
Hutagalung et al. (2011)
67 more

16 mentions without citation

Molecular control of Rab activity by GEFs, GAPs and GDI.
Müller et al. (2018)
15 more