› 3nma

X-ray diffraction
2.6Å resolution

Mutant P169S of Foot-and-mouth disease Virus RNA dependent RNA-polymerase

Released:
DOI: 10.2210/pdb3nma/pdb
PDB entry 3nma coloured by chain, front view.
PDB entry 3nma coloured by chain, side view.
PDB entry 3nma coloured by chain, top view.
Source organism: Foot and mouth disease virus C
Primary publication:
A multi-step process of viral adaptation to a mutagenic nucleoside analogue by modulation of transition types leads to extinction-escape.
Agudo R, Ferrer-Orta C, Arias A, de la Higuera I, Perales C, Pérez-Luque R, Verdaguer N, Domingo E
PLoS Pathog 6 e1001072 (2010)
PMID: 20865120

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234812 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Genome polyprotein Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 53.42 KDa
Source organism: Foot and mouth disease virus C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0QEE0 (Residues: 1525-1994; Coverage: 24%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:
5'-R(*GP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 3 nucleotides
Theoretical weight: 951 Da
Source organism: Foot and mouth disease virus C
Expression system: Not provided
5'-R(P*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 2 nucleotides
Theoretical weight: 565 Da
Source organism: Foot and mouth disease virus C
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P41212
Unit cell:
a: 93.834Å b: 93.834Å c: 121.687Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Viral quasispecies evolution.
Domingo et al. (2012)
18 more