› 3nmp

X-ray diffraction
2.1Å resolution

Crystal structure of the abscisic receptor PYL2 mutant A93F in complex with pyrabactin

Released:
DOI: 10.2210/pdb3nmp/pdb
PDB entry 3nmp coloured by chain, front view.
PDB entry 3nmp coloured by chain, side view.
PDB entry 3nmp coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Identification and mechanism of ABA receptor antagonism.
Melcher K, Xu Y, Ng LM, Zhou XE, Soon FF, Chinnusamy V, Suino-Powell KM, Kovach A, Tham FS, Cutler SR, Li J, Yong EL, Zhu JK, Xu HE
Nat Struct Mol Biol 17 1102-8 (2010)
PMID: 20729862

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131334 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Abscisic acid receptor PYL2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 178 amino acids
Theoretical weight: 20.1 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O80992 (Residues: 14-189; Coverage: 93%)
Gene names: At2g26040, PYL2, RCAR14, T19L18.15
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand PYV 3 x PYV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 62.084Å b: 105.571Å c: 182.901Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.266
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Advances and current challenges in calcium signaling.
Kudla et al. (2018)
18 more

2 mentions without citation

Chemical Manipulation of Abscisic Acid Signaling: A New Approach to Abiotic and Biotic Stress Management in Agriculture.
Hewage et al. (2020)
1 more