› 3nne

X-ray diffraction
2.47Å resolution

Crystal structure of choline oxidase S101A mutant

Released:
DOI: 10.2210/pdb3nne/pdb
PDB entry 3nne coloured by chain, front view.
PDB entry 3nne coloured by chain, side view.
PDB entry 3nne coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Structural and kinetic studies on the Ser101Ala variant of choline oxidase: catalysis by compromise.
Finnegan S, Yuan H, Wang YF, Orville AM, Weber IT, Gadda G
Arch Biochem Biophys 501 207-13 (2010)
PMID: 20561507

Function and Biology Details

Reaction catalysed: 
(1a) choline + O(2) = betaine aldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline oxidase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 546 amino acids
Theoretical weight: 59.88 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7X2H8 (Residues: 1-546; Coverage: 100%)
Gene name: codA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 8 x FAD
1 bound ligand:
Ligand ACT 8 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 69.258Å b: 346.029Å c: 105.919Å
α: 90° β: 94.33° γ: 90°
R-values:
R R work R free
0.231 0.228 0.293
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

The 1.6 Å crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate specificity and reveals a flavin intermediate.
Tan et al. (2013)
7 more

6 mentions without citation

Human choline dehydrogenase: medical promises and biochemical challenges.
Salvi et al. (2013)
5 more