› 3noy

X-ray diffraction
2.7Å resolution

Crystal structure of IspG (gcpE)

Released:
DOI: 10.2210/pdb3noy/pdb
PDB entry 3noy coloured by chain, front view.
PDB entry 3noy coloured by chain, side view.
PDB entry 3noy coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Biosynthesis of isoprenoids: crystal structure of the [4Fe-4S] cluster protein IspG.
Lee M, Gräwert T, Quitterer F, Rohdich F, Eppinger J, Eisenreich W, Bacher A, Groll M
J Mol Biol 404 600-10 (2010)
PMID: 20932974

Function and Biology Details

Reaction catalysed: 
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H(2)O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 366 amino acids
Theoretical weight: 40.29 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67496 (Residues: 1-357; Coverage: 100%)
Gene names: aq_1540, gcpE, ispG
Sequence domains: GcpE protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SF4 4 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 243.7Å b: 119.01Å c: 63.82Å
α: 90° β: 95.48° γ: 90°
R-values:
R R work R free
0.224 0.221 0.266
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Terpene biosynthesis: modularity rules.
Oldfield et al. (2012)
6 more

13 mentions without citation

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
12 more