› 3nyn

X-ray diffraction
2.72Å resolution

Crystal Structure of G Protein-Coupled Receptor Kinase 6 in Complex with Sangivamycin

Released:
DOI: 10.2210/pdb3nyn/pdb
PDB entry 3nyn coloured by chain, front view.
PDB entry 3nyn coloured by chain, side view.
PDB entry 3nyn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for activation of G protein-coupled receptor kinases.
Boguth CA, Singh P, Huang CC, Tesmer JJ
EMBO J 29 3249-59 (2010)
PMID: 20729810

Function and Biology Details

Reaction catalysed: 
ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-155004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G protein-coupled receptor kinase 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 576 amino acids
Theoretical weight: 65.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P43250 (Residues: 2-576; Coverage: 100%)
Gene names: GPRK6, GRK6
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SGV 2 x SGV
Ligand SO4 14 x SO4
Ligand BU3 2 x BU3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P61
Unit cell:
a: 154.59Å b: 154.59Å c: 207.914Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.238
Expression system: Trichoplusia ni

Quick links

Citations

25 review citations

G protein-coupled receptor kinases: more than just kinases and not only for GPCRs.
Gurevich et al. (2012)
24 more

22 mentions without citation

Recognition in the face of diversity: interactions of heterotrimeric G proteins and G protein-coupled receptor (GPCR) kinases with activated GPCRs.
Huang et al. (2011)
21 more