› 3nz4

X-ray diffraction
2.38Å resolution

Crystal Structure of a Taxus Phenylalanine Aminomutase

Released:
DOI: 10.2210/pdb3nz4/pdb
PDB entry 3nz4 coloured by chain, front view.
PDB entry 3nz4 coloured by chain, side view.
PDB entry 3nz4 coloured by chain, top view.
Source organism: Taxus canadensis
Primary publication:
Mechanistic, mutational, and structural evaluation of a Taxus phenylalanine aminomutase.
Feng L, Wanninayake U, Strom S, Geiger J, Walker KD
Biochemistry 50 2919-30 (2011)
PMID: 21361343

Function and Biology Details

Reactions catalysed: 
L-phenylalanine = trans-cinnamate + ammonia
L-phenylalanine = L-beta-phenylalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-179723 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine aminomutase (L-beta-phenylalanine forming) Chains: A, B
Molecule details ›
Chains: A, B
Length: 696 amino acids
Theoretical weight: 76.59 KDa
Source organism: Taxus canadensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6GZ04 (Residues: 1-698; Coverage: 100%)
Gene name: pam
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TCA 2 x TCA
1 modified residue:
Ligand MDO 2 x MDO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 181.733Å b: 76.113Å c: 120.392Å
α: 90° β: 120.43° γ: 90°
R-values:
R R work R free
0.189 0.183 0.239
Expression system: Escherichia coli

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Citations

4 review citations

New generation of biocatalysts for organic synthesis.
Nestl et al. (2014)
3 more

2 mentions without citation

A Methylidene Group in the Phosphonic Acid Analogue of Phenylalanine Reverses the Enantiopreference of Binding to Phenylalanine Ammonia-Lyases.
Bata et al. (2017)
1 more