› 3o12

X-ray diffraction
1.5Å resolution

The crystal structure of a functionally unknown protein from Saccharomyces cerevisiae.

Released:
DOI: 10.2210/pdb3o12/pdb
PDB entry 3o12 coloured by chain, front view.
PDB entry 3o12 coloured by chain, side view.
PDB entry 3o12 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Zhang R, Tan K, Xu X, Cui H, Chin S, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulation of enolase protein 1 Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P40893 (Residues: 1-198; Coverage: 100%)
Gene names: HRC198, J0226, REE1, YJL217W
Sequence domains: Protein of unknown function (DUF1349)
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand EDO 4 x EDO
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 112.665Å b: 112.665Å c: 92.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.167 0.185
Expression system: Escherichia coli BL21

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