› 3o33

X-ray diffraction
2Å resolution

Crystal structure of TRIM24 PHD-Bromo in the free state

Released:
DOI: 10.2210/pdb3o33/pdb
PDB entry 3o33 coloured by chain, front view.
PDB entry 3o33 coloured by chain, side view.
PDB entry 3o33 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
TRIM24 links a non-canonical histone signature to breast cancer.
Tsai WW, Wang Z, Yiu TT, Akdemir KC, Xia W, Winter S, Tsai CY, Shi X, Schwarzer D, Plunkett W, Aronow B, Gozani O, Fischle W, Hung MC, Patel DJ, Barton MC
Nature 468 927-32 (2010)
PMID: 21164480

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription intermediary factor 1-alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 184 amino acids
Theoretical weight: 21.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15164 (Residues: 824-1006; Coverage: 17%)
Gene names: RNF82, TIF1, TIF1A, TRIM24
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 36.482Å b: 48.498Å c: 122.729Å
α: 86.45° β: 81.46° γ: 67.84°
R-values:
R R work R free
0.221 0.221 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

71 review citations

Histone methylation: a dynamic mark in health, disease and inheritance.
Greer et al. (2012)
70 more

6 mentions without citation

TRIM24 links a non-canonical histone signature to breast cancer.
Tsai et al. (2010)
5 more