› 3o3c

X-ray diffraction
3.51Å resolution

Glycogen synthase basal state UDP complex

Released:
DOI: 10.2210/pdb3o3c/pdb
PDB entry 3o3c coloured by chain, front view.
PDB entry 3o3c coloured by chain, side view.
PDB entry 3o3c coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for glucose-6-phosphate activation of glycogen synthase.
Baskaran S, Roach PJ, DePaoli-Roach AA, Hurley TD
Proc Natl Acad Sci U S A 107 17563-8 (2010)
PMID: 20876143

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen [starch] synthase isoform 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 725 amino acids
Theoretical weight: 82.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P27472 (Residues: 1-705; Coverage: 100%)
Gene names: GSY2, L8479.8, YLR258W
Sequence domains: Glycogen synthase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand SO4 11 x SO4
Ligand UDP 4 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 96.586Å b: 167.209Å c: 121.251Å
α: 90° β: 102.66° γ: 90°
R-values:
R R work R free
0.221 0.219 0.249
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Glycogen and its metabolism: some new developments and old themes.
Roach et al. (2012)
10 more

1 mention without citation

Structural basis for glucose-6-phosphate activation of glycogen synthase.
Baskaran et al. (2010)