› 3o44

X-ray diffraction
2.88Å resolution

Crystal Structure of the Vibrio cholerae Cytolysin (HlyA) Heptameric Pore

Released:
DOI: 10.2210/pdb3o44/pdb
PDB entry 3o44 coloured by chain, front view.
PDB entry 3o44 coloured by chain, side view.
PDB entry 3o44 coloured by chain, top view.
Source organism: Vibrio cholerae 12129(1)
Primary publication:
Crystal structure of the Vibrio cholerae cytolysin heptamer reveals common features among disparate pore-forming toxins.
De S, Olson R
Proc Natl Acad Sci U S A 108 7385-90 (2011)
PMID: 21502531

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemolysin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 593 amino acids
Theoretical weight: 65.72 KDa
Source organism: Vibrio cholerae 12129(1)
Expression system: Escherichia coli
UniProt:
  • Canonical: P09545 (Residues: 149-741; Coverage: 83%)
Gene names: VC_A0219, hlyA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 172.354Å b: 182.859Å c: 430.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.249
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Role of pore-forming toxins in bacterial infectious diseases.
Los et al. (2013)
14 more

15 mentions without citation

Signaling beyond Punching Holes: Modulation of Cellular Responses by Vibrio cholerae Cytolysin.
Khilwani et al. (2015)
14 more