› 3o5y

X-ray diffraction
2.45Å resolution

The Crystal Structure of the GAF domain of a two-component sensor histidine kinase from Bacillus halodurans to 2.45A

Released:
DOI: 10.2210/pdb3o5y/pdb
PDB entry 3o5y coloured by chain, front view.
PDB entry 3o5y coloured by chain, side view.
PDB entry 3o5y coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Entry authors: Stein AJ, Mack J, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-191858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 165 amino acids
Theoretical weight: 19.16 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KFE5 (Residues: 8-169; Coverage: 32%)
Gene name: cckA
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand ACT 3 x ACT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P4122
Unit cell:
a: 96.961Å b: 96.961Å c: 104.695Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.269
Expression system: Escherichia coli

Quick links