› 3o9m

X-ray diffraction
2.98Å resolution

Co-crystallization studies of full length recombinant BChE with cocaine offers insights into cocaine detoxification

Released:
DOI: 10.2210/pdb3o9m/pdb
PDB entry 3o9m coloured by chain, front view.
PDB entry 3o9m coloured by chain, side view.
PDB entry 3o9m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cocrystallization studies of full-length recombinant butyrylcholinesterase (BChE) with cocaine.
Asojo OA, Asojo OA, Ngamelue MN, Homma K, Lockridge O
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 434-7 (2011)
PMID: 21505234

Function and Biology Details

Reaction catalysed: 
An acylcholine + H(2)O = choline + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-138984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholinesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 65.15 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06276 (Residues: 29-602; Coverage: 100%)
Gene names: BCHE, CHE1
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand BEZ 2 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P4212
Unit cell:
a: 150.392Å b: 150.392Å c: 142.448Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.262
Expression system: Cricetulus griseus

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Citations

6 mentions without citation

Plasma butyrylcholinesterase regulates ghrelin to control aggression.
Chen et al. (2015)
5 more