› 3odz

X-ray diffraction
2.3Å resolution

Crystal structure of P38alpha Y323R active mutant

Released:
DOI: 10.2210/pdb3odz/pdb
PDB entry 3odz coloured by chain, front view.
PDB entry 3odz coloured by chain, side view.
PDB entry 3odz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Active mutants of the TCR-mediated p38α alternative activation site show changes in the phosphorylation lip and DEF site formation.
Tzarum N, Diskin R, Engelberg D, Livnah O
J Mol Biol 405 1154-69 (2011)
PMID: 21146537

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 14 Chain: X
Molecule details ›
Chain: X
Length: 360 amino acids
Theoretical weight: 41.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16539 (Residues: 1-360; Coverage: 100%)
Gene names: CSBP, CSBP1, CSBP2, CSPB1, MAPK14, MXI2, SAPK2A
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BOG 2 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 40.047Å b: 72.313Å c: 68.963Å
α: 90° β: 105.02° γ: 90°
R-values:
R R work R free
0.223 0.214 0.282
Expression system: Escherichia coli

Quick links

Citations

1 review citation

A Comprehensive Structural Overview of p38α Mitogen-Activated Protein Kinase in Complex with ATP-Site and Non-ATP-Site Binders.
Astolfi et al. (2018)
11 other articles

2 mentions without citation

Conformational bias imposed by source microseeds results in structural ambiguity.
Tzarum et al. (2011)
1 more