› 3og7

X-ray diffraction
2.45Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 32.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15056 (Residues: 449-720; Coverage: 36%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 032 1 x 032
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 50.77Å b: 104.424Å c: 110.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.258
Expression system: Escherichia coli

Quick links

Citations

329 review citations

ERK1/2 MAP kinases: structure, function, and regulation.
Roskoski R. (2012)
328 more

69 mentions without citation

Ten things you should know about protein kinases: IUPHAR Review 14.
Fabbro et al. (2015)
68 more