› 3oj0

X-ray diffraction
1.65Å resolution

Crystal structure of glutamyl-tRNA reductase from Thermoplasma volcanium (nucleotide binding domain)

Released:
DOI: 10.2210/pdb3oj0/pdb
PDB entry 3oj0 coloured by chain, front view.
PDB entry 3oj0 coloured by chain, side view.
PDB entry 3oj0 coloured by chain, top view.
Source organism: Thermoplasma volcanium GSS1
Entry authors: Michalska K, Marshall N, Clancy S, Puttagunta R, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
L-glutamate 1-semialdehyde + NADP(+) + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamyl-tRNA reductase Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.34 KDa
Source organism: Thermoplasma volcanium GSS1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q97B68 (Residues: 141-281; Coverage: 35%)
Gene names: TV0590, TVG0579613, hemA
Sequence domains: Shikimate / quinate 5-dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P61
Unit cell:
a: 92.52Å b: 92.52Å c: 33.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.183
Expression system: Escherichia coli BL21

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