› 3oja

X-ray diffraction
2.7Å resolution

Crystal structure of LRIM1/APL1C complex

Released:
DOI: 10.2210/pdb3oja/pdb
PDB entry 3oja coloured by chain, front view.
PDB entry 3oja coloured by chain, side view.
PDB entry 3oja coloured by chain, top view.
Source organism: Anopheles gambiae
Primary publication:
A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae.
Baxter RH, Steinert S, Chelliah Y, Volohonsky G, Levashina EA, Deisenhofer J
Proc Natl Acad Sci U S A 107 16817-22 (2010)
PMID: 20826443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163858 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
LRIM1/APL1C-like dimerization domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 487 amino acids
Theoretical weight: 55.1 KDa
Source organism: Anopheles gambiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q7Q5N3 (Residues: 23-509; Coverage: 100%)
Gene names: 1276956, AgaP_AGAP006348
Sequence domains:
Structure domains:
Anopheles Plasmodium-responsive Leucine-rich repeat protein 1 Chain: B
Molecule details ›
Chain: B
Length: 597 amino acids
Theoretical weight: 69.19 KDa
Source organism: Anopheles gambiae
Expression system: Trichoplusia ni
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 109.89Å b: 110.89Å c: 168.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.265
Expression system: Trichoplusia ni

Quick links

Citations

5 review citations

The Anopheles innate immune system in the defense against malaria infection.
Clayton et al. (2014)
4 more

5 mentions without citation

A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae.
Baxter et al. (2010)
4 more