› 3okc

X-ray diffraction
2.4Å resolution

Crystal structure of Corynebacterium glutamicum PimB' bound to GDP (orthorhombic crystal form)

Released:
DOI: 10.2210/pdb3okc/pdb
PDB entry 3okc coloured by chain, front view.
PDB entry 3okc coloured by chain, side view.
PDB entry 3okc coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Primary publication:
Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB'.
Batt SM, Jabeen T, Mishra AK, Veerapen N, Krumbach K, Eggeling L, Besra GS, Fütterer K
J Biol Chem 285 37741-52 (2010)
PMID: 20843801

Function and Biology Details

Reaction catalysed: 
GDP-alpha-D-mannose + 2-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol = GDP + 2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185614 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-mannose-dependent monoacylated alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 42.9 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NNK8 (Residues: 1-381; Coverage: 100%)
Gene names: Cgl2186, cg2400, pimB
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 44.852Å b: 86.4Å c: 106.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.236
Expression system: Escherichia coli

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Citations

6 review citations

Lipoarabinomannan and related glycoconjugates: structure, biogenesis and role in Mycobacterium tuberculosis physiology and host-pathogen interaction.
Mishra et al. (2011)
5 more

1 mention without citation

Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB'.
Batt et al. (2010)