› 3ol7

X-ray diffraction
2.7Å resolution

Poliovirus polymerase elongation complex with CTP

Released:
DOI: 10.2210/pdb3ol7/pdb
PDB entry 3ol7 coloured by chain, front view.
PDB entry 3ol7 coloured by chain, side view.
PDB entry 3ol7 coloured by chain, top view.
Source organism: Poliovirus 1
Primary publication:
Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase.
Gong P, Peersen OB
Proc Natl Acad Sci U S A 107 22505-10 (2010)
PMID: 21148772

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136691 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct RNA molecules
Macromolecules (4 distinct):
RNA-directed RNA polymerase Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 471 amino acids
Theoretical weight: 53.76 KDa
Source organism: Human poliovirus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03300 (Residues: 1749-2209; Coverage: 21%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:
RNA (5'-R(*AP*AP*GP*UP*CP*UP*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*GP*UP*CP*CP*GP*GP*AP*AP*A)-3') Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 26 nucleotides
Theoretical weight: 8.28 KDa
Source organism: Poliovirus 1
Expression system: Not provided
RNA (5'-R(*GP*CP*CP*CP*GP*GP*AP*CP*GP*AP*GP*AP*GP*AP*C)-3') Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 15 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Poliovirus 1
Expression system: Not provided
RNA (5'-R(*GP*GP*GP*AP*GP*AP*UP*GP*A)-3') Chains: D, H, L, P
Molecule details ›
Chains: D, H, L, P
Length: 9 nucleotides
Theoretical weight: 2.97 KDa
Source organism: Poliovirus 1
Expression system: Not provided

Ligands and Environments

6 bound ligands:
Ligand MG 6 x MG
Ligand POP 4 x POP
Ligand IPA 15 x IPA
Ligand PEG 4 x PEG
Ligand ZN 4 x ZN
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P1
Unit cell:
a: 60.724Å b: 60.769Å c: 192.969Å
α: 83.01° β: 82.93° γ: 77.12°
R-values:
R R work R free
0.229 0.226 0.29
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

41 review citations

Mechanisms of viral mutation.
Sanjuán et al. (2016)
40 more

25 mentions without citation

Common and unique features of viral RNA-dependent polymerases.
te Velthuis AJ. (2014)
24 more