› 3olo

X-ray diffraction
2.09Å resolution

Crystal structure of a PAS domain from two-component sensor histidine kinase

Released:
DOI: 10.2210/pdb3olo/pdb
PDB entry 3olo coloured by chain, front view.
PDB entry 3olo coloured by chain, side view.
PDB entry 3olo coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Michalska K, Chhor G, Bearden J, Fenske RJ, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 118 amino acids
Theoretical weight: 14.38 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8YZM9 (Residues: 1-115; Coverage: 18%)
Gene name: alr0428
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 85.43Å b: 85.43Å c: 67.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.227
Expression system: Escherichia coli BL21

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