› 3omw

X-ray diffraction
2.87Å resolution

Crystal structure of Ssu72, an essential eukaryotic phosphatase specific for the C-terminal domain of RNA polymerase II

Released:
DOI: 10.2210/pdb3omw/pdb
PDB entry 3omw coloured by chain, front view.
PDB entry 3omw coloured by chain, side view.
PDB entry 3omw coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of Ssu72, an essential eukaryotic phosphatase specific for the C-terminal domain of RNA polymerase II, in complex with a transition state analogue.
Zhang Y, Zhang M, Zhang Y
Biochem J 434 435-44 (2011)
PMID: 21204787

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase II subunit A C-terminal domain phosphatase SSU72 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 22.23 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VWE4 (Residues: 6-195; Coverage: 97%)
Gene names: BEST:CK01830, CG14216, Dmel\CG14216, Dmel_CG14216, Ssu72, dSsu72
Sequence domains: Ssu72-like protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21212
Unit cell:
a: 158.1Å b: 101.929Å c: 65.645Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Protein tyrosine phosphatases--from housekeeping enzymes to master regulators of signal transduction.
Tonks NK. (2013)
6 more

3 mentions without citation

novel modifications on C-terminal domain of RNA polymerase II can fine-tune the phosphatase activity of Ssu72.
Luo et al. (2013)
2 more