› 3ork

X-ray diffraction
1.6Å resolution

Mycobacterium tuberculosis PknB kinase domain L33D mutant (crystal form 2)

Released:
DOI: 10.2210/pdb3ork/pdb
PDB entry 3ork coloured by chain, front view.
PDB entry 3ork coloured by chain, side view.
PDB entry 3ork coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Ra
Primary publication:
Allosteric activation mechanism of the Mycobacterium tuberculosis receptor Ser/Thr kinase, PknB
Lombana TN, Echols N, Good MC, Thomsen ND, Ng H-L, Greenstein AE, Falick AM, King DS, Alber T
Structure 18 1667-1677 (2010)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknB Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 33.52 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WI81 (Residues: 1-308; Coverage: 49%)
Gene names: MTCY10H4.14c, Rv0014c, pknB
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand AGS 1 x AGS
Ligand MN 1 x MN
Ligand SO4 1 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 39.341Å b: 51.603Å c: 155.126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.18
Expression system: Escherichia coli

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