› 3osu

X-ray diffraction
1.9Å resolution

Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, FabG, from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3osu/pdb
PDB entry 3osu coloured by chain, front view.
PDB entry 3osu coloured by chain, side view.
PDB entry 3osu coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Entry authors: Anderson SM, Wawrzak Z, Onopriyenko O, Edwards A, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase FabG Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 26.64 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A0H9 (Residues: 1-246; Coverage: 100%)
Gene names: SAV1231, fabG
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand PEG 14 x PEG
Ligand MG 2 x MG
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3121
Unit cell:
a: 64.06Å b: 64.06Å c: 183.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.157 0.193
Expression system: Escherichia coli BL21(DE3)

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