› 3otf

X-ray diffraction
2.4Å resolution

Structural basis for the cAMP-dependent gating in human HCN4 channel

Released:
DOI: 10.2210/pdb3otf/pdb
PDB entry 3otf coloured by chain, front view.
PDB entry 3otf coloured by chain, side view.
PDB entry 3otf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the cAMP-dependent gating in the human HCN4 channel.
Xu X, Vysotskaya ZV, Liu Q, Zhou L
J Biol Chem 285 37082-91 (2010)
PMID: 20829353

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 25.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3Q4 (Residues: 521-739; Coverage: 18%)
Gene name: HCN4
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CMP 1 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: I422
Unit cell:
a: 69.264Å b: 69.264Å c: 191.616Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.237 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Exploring HCN channels as novel drug targets.
Postea et al. (2011)
7 more

16 mentions without citation

Structural basis for the cAMP-dependent gating in the human HCN4 channel.
Xu et al. (2010)
15 more