› 3oup

X-ray diffraction
1.65Å resolution

Crystal structure of the gamma-carbonic anhydrase W19N mutant from Methanosarcina thermophila

Released:
DOI: 10.2210/pdb3oup/pdb
PDB entry 3oup coloured by chain, front view.
PDB entry 3oup coloured by chain, side view.
PDB entry 3oup coloured by chain, top view.
Source organism: Methanosarcina thermophila
Entry authors: Domsic JF, Robbins AH, McKenna R

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 22.82 KDa
Source organism: Methanosarcina thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P40881 (Residues: 35-247; Coverage: 100%)
Gene name: MSTHT_0588
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P213
Unit cell:
a: 83.563Å b: 83.563Å c: 83.563Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.137 0.158
Expression system: Escherichia coli

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