› 3p11

X-ray diffraction
3.7Å resolution

anti-EGFR/HER3 Fab DL11 in complex with domains I-III of the HER3 extracellular region

Released:
DOI: 10.2210/pdb3p11/pdb
PDB entry 3p11 coloured by chain, front view.
PDB entry 3p11 coloured by chain, side view.
PDB entry 3p11 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A two-in-one antibody against HER3 and EGFR has superior inhibitory activity compared with monospecific antibodies.
Schaefer G, Haber L, Crocker LM, Shia S, Shao L, Dowbenko D, Totpal K, Wong A, Lee CV, Stawicki S, Clark R, Fields C, Lewis Phillips GD, Prell RA, Danilenko DM, Franke Y, Stephan JP, Hwang J, Wu Y, Bostrom J, Sliwkowski MX, Fuh G, Eigenbrot C
Cancer Cell 20 472-86 (2011)
PMID: 22014573

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210840 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Fab DL11 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab DL11 light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-3 Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 57.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21860 (Residues: 20-532; Coverage: 39%)
Gene names: ERBB3, HER3
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 208.392Å b: 48.397Å c: 130.381Å
α: 90° β: 127.71° γ: 90°
R-values:
R R work R free
0.249 0.245 0.323
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

67 review citations

ERBB receptors: from oncogene discovery to basic science to mechanism-based cancer therapeutics.
Arteaga et al. (2014)
66 more

25 mentions without citation

The molecular basis of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum.
Van Langenhove et al. (2012)
24 more