› 3p1o

X-ray diffraction
1.9Å resolution

Crystal structure of human 14-3-3 sigma in complex with TASK-3 peptide and stabilisator Fusicoccin A

Released:
DOI: 10.2210/pdb3p1o/pdb
PDB entry 3p1o coloured by chain, front view.
PDB entry 3p1o coloured by chain, side view.
PDB entry 3p1o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A semisynthetic fusicoccane stabilizes a protein-protein interaction and enhances the expression of K+ channels at the cell surface.
Anders C, Higuchi Y, Koschinsky K, Bartel M, Schumacher B, Thiel P, Nitta H, Preisig-Müller R, Schlichthörl G, Renigunta V, Ohkanda J, Daut J, Kato N, Ottmann C
Chem Biol 20 583-93 (2013)
PMID: 23601647

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152170 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Potassium channel subfamily K member 9 Chain: P
Molecule details ›
Chain: P
Length: 6 amino acids
Theoretical weight: 857 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NPC2 (Residues: 369-374; Coverage: 2%)
Gene names: KCNK9, TASK3

Ligands and Environments

4 bound ligands:
Ligand FSC 1 x FSC
Ligand CL 1 x CL
Ligand CA 1 x CA
Ligand MG 4 x MG
2 modified residues:
Ligand CSO 1 x CSO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2221
Unit cell:
a: 84.1Å b: 109.74Å c: 62.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Modulators of 14-3-3 Protein-Protein Interactions.
Stevers et al. (2018)
16 more

1 mention without citation

Protein-protein interfaces in molecular glue-induced ternary complexes: classification, characterization, and prediction.
Rui et al. (2023)