› 3p4f

X-ray diffraction
2.35Å resolution

Structural and biochemical insights into MLL1 core complex assembly and regulation.

Released:
DOI: 10.2210/pdb3p4f/pdb
PDB entry 3p4f coloured by chain, front view.
PDB entry 3p4f coloured by chain, side view.
PDB entry 3p4f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical insights into MLL1 core complex assembly.
Avdic V, Zhang P, Lanouette S, Groulx A, Tremblay V, Brunzelle J, Couture JF
Structure 19 101-8 (2011)
PMID: 21220120

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158495 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 34.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 22-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Retinoblastoma-binding protein 5 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15291 (Residues: 371-381; Coverage: 2%)
Gene names: RBBP5, RBQ3
MLL cleavage product C180 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q03164 (Residues: 3761-3770; Coverage: 0%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 53.85Å b: 81.35Å c: 81.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.249
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Hijacked in cancer: the KMT2 (MLL) family of methyltransferases.
Rao et al. (2015)
16 more

8 mentions without citation

Structure, function and inhibition of critical protein-protein interactions involving mixed lineage leukemia 1 and its fusion oncoproteins.
Li et al. (2021)
7 more