Function and Biology Details
Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128154 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase sliding clamp
Molecule details ›
Chains: A, B, C
Length: 245 amino acids
Theoretical weight: 27.3 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 245 amino acids
Theoretical weight: 27.3 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
O29912 (Residues: 1-245; Coverage: 100%)
Sequence domains:
- Proliferating cell nuclear antigen, N-terminal domain
- Proliferating cell nuclear antigen, C-terminal domain
Ribonuclease HII
Molecule details ›
Chains: D, E, F
Length: 217 amino acids
Theoretical weight: 24.43 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ribonuclease HII
Structure domains:
Length: 217 amino acids
Theoretical weight: 24.43 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: O29634 (Residues: 1-205; Coverage: 100%)
Sequence domains: Ribonuclease HII
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
DIAMOND BEAMLINE I04
Spacegroup:
P21212
Expression system: Escherichia coli BL21(DE3)
