› 3pdb

X-ray diffraction
2.4Å resolution

Crystal structure of mouse mitochondrial aspartate aminotransferase in complex with oxaloacetic acid

Released:
DOI: 10.2210/pdb3pdb/pdb
PDB entry 3pdb coloured by chain, front view.
PDB entry 3pdb coloured by chain, side view.
PDB entry 3pdb coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Biochemical and structural characterization of mouse mitochondrial aspartate aminotransferase, a newly identified kynurenine aminotransferase-IV.
Han Q, Robinson H, Cai T, Tagle DA, Li J
Biosci Rep 31 323-32 (2011)
PMID: 20977429

Function and Biology Details

Reactions catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138594 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartate aminotransferase, mitochondrial Chains: A, C
Molecule details ›
Chains: A, C
Length: 401 amino acids
Theoretical weight: 44.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05202 (Residues: 30-430; Coverage: 93%)
Gene names: Got-2, Got2
Sequence domains: Aminotransferase class I and II
Structure domains:
Aspartate aminotransferase, mitochondrial Chains: B, D
Molecule details ›
Chains: B, D
Length: 401 amino acids
Theoretical weight: 44.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05202 (Residues: 30-430; Coverage: 93%)
Gene names: Got-2, Got2
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 2 x PMP
3 bound ligands:
Ligand BME 7 x BME
Ligand GOL 5 x GOL
Ligand OAA 1 x OAA
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 282.418Å b: 77.894Å c: 87.389Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Inflammation, vitamin B6 and related pathways.
Ueland et al. (2017)
5 more

11 mentions without citation

The Synthesis of Kynurenic Acid in Mammals: An Updated Kynurenine Aminotransferase Structural KATalogue.
Rossi et al. (2019)
10 more