› 3pe3

X-ray diffraction
2.78Å resolution

Structure of human O-GlcNAc transferase and its complex with a peptide substrate

Released:
DOI: 10.2210/pdb3pe3/pdb
PDB entry 3pe3 coloured by chain, front view.
PDB entry 3pe3 coloured by chain, side view.
PDB entry 3pe3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human O-GlcNAc transferase and its complex with a peptide substrate.
Lazarus MB, Nam Y, Jiang J, Sliz P, Walker S
Nature 469 564-7 (2011)
PMID: 21240259

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 723 amino acids
Theoretical weight: 80.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15294 (Residues: 323-1041; Coverage: 69%)
Gene name: OGT
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UDP 4 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P321
Unit cell:
a: 273.4Å b: 273.4Å c: 142.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 0.218
Expression system: Escherichia coli

Quick links

Citations

79 review citations

Bittersweet memories: linking metabolism to epigenetics through O-GlcNAcylation.
Hanover et al. (2012)
78 more

13 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
12 more