› 3phz

X-ray diffraction
1.7Å resolution

Crystal Structure Analysis of Polyporus squamosus lectin bound to human-type influenza-binding epitope Neu5Aca2-6Galb1-4GlcNAc

Released:
DOI: 10.2210/pdb3phz/pdb
PDB entry 3phz coloured by chain, front view.
PDB entry 3phz coloured by chain, side view.
PDB entry 3phz coloured by chain, top view.
Source organism: Cerioporus squamosus
Primary publication:
Structure and binding analysis of Polyporus squamosus lectin in complex with the Neu5Ac{alpha}2-6Gal{beta}1-4GlcNAc human-type influenza receptor.
Kadirvelraj R, Grant OC, Goldstein IJ, Winter HC, Tateno H, Fadda E, Woods RJ
Glycobiology 21 973-84 (2011)
PMID: 21436237

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181131 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Ricin B-related lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 31.21 KDa
Source organism: Cerioporus squamosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WT9 (Residues: 1-286; Coverage: 100%)
Gene name: psl1a
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: SIA, NAG, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL
Carbohydrate polymer
1 bound ligand:
Ligand SIA 1 x SIA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21212
Unit cell:
a: 115.809Å b: 59.4Å c: 103.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.217
Expression system: Escherichia coli

Quick links

Citations

9 review citations

A guide into glycosciences: How chemistry, biochemistry and biology cooperate to crack the sugar code.
Solís et al. (2015)
8 more

3 mentions without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
2 more