› 3pjb

X-ray diffraction
1.75Å resolution

Crystal structure of red fluorescent protein eqFP578 crystallized at pH 4.0

Released:
DOI: 10.2210/pdb3pjb/pdb
PDB entry 3pjb coloured by chain, front view.
PDB entry 3pjb coloured by chain, side view.
PDB entry 3pjb coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore.
Pletneva NV, Pletnev VZ, Shemiakina II, Chudakov DM, Artemyev I, Wlodawer A, Dauter Z, Pletnev S
Protein Sci 20 1265-74 (2011)
PMID: 21563226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein eqFP578 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.09 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
UniProt:
  • Canonical: H3JQU7 (Residues: 1-231; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand NRQ 2 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 104.658Å b: 104.658Å c: 216.527Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.176 0.208
Expression system: Escherichia coli

Quick links

Citations

14 citation in other articles

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Akerboom et al. (2013)
13 more

1 mention without citation

Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore.
Pletneva et al. (2011)