› 3pni

X-ray diffraction
2.8Å resolution

Crystal structure of D14C [3Fe-4S] Pyrococcus furiosus ferredoxin

Released:
DOI: 10.2210/pdb3pni/pdb
PDB entry 3pni coloured by chain, front view.
PDB entry 3pni coloured by chain, side view.
PDB entry 3pni coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structures of the all-cysteinyl-coordinated D14C variant of Pyrococcus furiosus ferredoxin: [4Fe-4S] ↔ [3Fe-4S] cluster conversion.
Løvgreen MN, Martic M, Windahl MS, Christensen HE, Harris P
J Biol Inorg Chem 16 763-75 (2011)
PMID: 21484348

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.16 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29603 (Residues: 2-67; Coverage: 99%)
Gene names: PF1909, fdxA
Sequence domains: 4Fe-4S single cluster domain of Ferredoxin I
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand F3S 2 x F3S
Ligand CO 4 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 47.4Å b: 49.8Å c: 51.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.281 0.279 0.318
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Heterometallic [AgFe(3)S (4)] ferredoxin variants: synthesis, characterization, and the first crystal structure of an engineered heterometallic iron-sulfur protein.
Martic et al. (2013)
2 more

1 mention without citation

Characterization of the [3Fe-4S](0/1+) cluster from the D14C variant of Pyrococcus furiosus ferredoxin via combined NRVS and DFT analyses.
Lauterbach et al. (2016)