› 3pps

X-ray diffraction
2.5Å resolution

Crystal structure of an ascomycete fungal laccase from Thielavia arenaria

Released:
DOI: 10.2210/pdb3pps/pdb
PDB entry 3pps coloured by chain, front view.
PDB entry 3pps coloured by chain, side view.
PDB entry 3pps coloured by chain, top view.
Source organism: Canariomyces arenarius
Primary publication:
Crystal structure of an ascomycete fungal laccase from Thielavia arenaria--common structural features of asco-laccases.
Kallio JP, Gasparetti C, Andberg M, Boer H, Koivula A, Kruus K, Rouvinen J, Hakulinen N
FEBS J 278 2283-95 (2011)
PMID: 21535408

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
laccase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 604 amino acids
Theoretical weight: 66.6 KDa
Source organism: Canariomyces arenarius
Expression system: Trichoderma reesei QM6a
UniProt:
  • Canonical: F6N9E7 (Residues: 1-604; Coverage: 100%)
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand CU 16 x CU
Ligand OXY 1 x OXY
Ligand NAG 8 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 61.36Å b: 178.95Å c: 118.13Å
α: 90° β: 90.26° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Trichoderma reesei QM6a

Quick links

Citations

8 review citations

Laccase immobilization and insolubilization: from fundamentals to applications for the elimination of emerging contaminants in wastewater treatment.
Ba et al. (2013)
7 more

8 mentions without citation

Structural changes caused by radiation-induced reduction and radiolysis: the effect of X-ray absorbed dose in a fungal multicopper oxidase.
De la Mora et al. (2012)
7 more