› 3ppx

X-ray diffraction
1.91Å resolution

Crystal structure of the N1602A mutant of an engineered VWF A2 domain (N1493C and C1670S)

Released:
DOI: 10.2210/pdb3ppx/pdb
PDB entry 3ppx coloured by chain, front view.
PDB entry 3ppx coloured by chain, side view.
PDB entry 3ppx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel calcium-binding site of von Willebrand factor A2 domain regulates its cleavage by ADAMTS13.
Zhou M, Dong X, Baldauf C, Chen H, Zhou Y, Springer TA, Luo X, Zhong C, Gräter F, Ding J
Blood 117 4623-31 (2011)
PMID: 21385852

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
von Willebrand factor Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04275 (Residues: 1488-1674; Coverage: 7%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P65
Unit cell:
a: 72.086Å b: 72.086Å c: 76.344Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.237
Expression system: Escherichia coli

Quick links

Citations

4 review citations

von Willebrand factor, Jedi knight of the bloodstream.
Springer TA. (2014)
3 more