3pvv

X-ray diffraction
2Å resolution

Structure of Mycobacterium tuberculosis DnaA-DBD in complex with box1 DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-107719 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Chromosomal replication initiator protein DnaA Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.57 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli
UniProt:
  • Canonical: A5TY69 (Residues: 411-507; Coverage: 19%)
Gene names: MRA_0001, dnaA
Sequence domains: Bacterial dnaA protein helix-turn-helix
Structure domains: Chromosomal Replication Initiator Protein Dnaa; Chain: A;
DNA (5'-D(*CP*GP*TP*TP*GP*TP*CP*CP*AP*CP*AP*AP*C)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 13 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Not provided
DNA (5'-D(*GP*TP*TP*GP*TP*GP*GP*AP*CP*AP*AP*CP*G)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 13 nucleotides
Theoretical weight: 4.03 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3221
Unit cell:
a: 88.441Å b: 88.441Å c: 105.156Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.251 0.271
Expression systems:
  • Escherichia coli
  • Not provided