› 3pxu

X-ray diffraction
2.1Å resolution

Crystal structure of phosphopantetheine adenylyltransferase from Burkholderia pseudomallei bound to dephospho-coenzyme A

Released:
DOI: 10.2210/pdb3pxu/pdb
PDB entry 3pxu coloured by chain, front view.
PDB entry 3pxu coloured by chain, side view.
PDB entry 3pxu coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Primary publication:
Structures of phosphopantetheine adenylyltransferase from Burkholderia pseudomallei.
Edwards TE, Leibly DJ, Bhandari J, Statnekov JB, Phan I, Dieterich SH, Abendroth J, Staker BL, Van Voorhis WC, Myler PJ, Stewart LJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1032-7 (2011)
PMID: 21904046

Function and Biology Details

Reaction catalysed: 
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer (preferred)
homo 24-mer
Assembly name:
PDBe Complex ID:
PDB-CPX-174689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantetheine adenylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 18.85 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JW91 (Residues: 1-166; Coverage: 100%)
Gene names: BURPS1710b_0748, coaD
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

3 bound ligands:
Ligand COD 1 x COD
Ligand GOL 2 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: I432
Unit cell:
a: 134.161Å b: 134.161Å c: 134.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural Biology and the Design of New Therapeutics: From HIV and Cancer to Mycobacterial Infections: A Paper Dedicated to John Kendrew.
Thomas et al. (2017)
2 other articles

2 mentions without citation

Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh et al. (2013)
1 more