› 3py3

X-ray diffraction
2.1Å resolution

Crystal structure of phosphorylated p38alpha MAP kinase

Released:
DOI: 10.2210/pdb3py3/pdb
PDB entry 3py3 coloured by chain, front view.
PDB entry 3py3 coloured by chain, side view.
PDB entry 3py3 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Mitogen-activated protein kinase (MAPK) phosphatase 3-mediated cross-talk between MAPKs ERK2 and p38alpha.
Zhang YY, Wu JW, Wang ZX
J Biol Chem 286 16150-62 (2011)
PMID: 21454500

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 14 Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 43.67 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47811 (Residues: 1-360; Coverage: 100%)
Gene names: Crk1, Csbp1, Csbp2, Mapk14
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand TPO 1 x TPO
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 47.272Å b: 52.284Å c: 168.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.184 0.24
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Dual-specificity MAP kinase phosphatases (MKPs): shaping the outcome of MAP kinase signalling.
Caunt et al. (2013)
3 more

18 mentions without citation

Design principles underpinning the regulatory diversity of protein kinases.
Oruganty et al. (2012)
17 more