3q0q

X-ray diffraction
2Å resolution

Crystal structure of the PUMILIO-homology domain from Human PUMILIO2 in complex with p38alpha NREa

Released:
Source organism: Homo sapiens
Primary publication:
Alternate modes of cognate RNA recognition by human PUMILIO proteins.
Structure 19 361-7 (2011)
PMID: 21397187

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-186169 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pumilio homolog 2 Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TB72 (Residues: 706-1056; Coverage: 33%)
Gene names: KIAA0235, PUM2, PUMH2
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(UP*GP*UP*AP*AP*AP*UP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.54 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 36.438Å b: 43.03Å c: 60.845Å
α: 71.61° β: 85.73° γ: 77.99°
R-values:
R R work R free
0.197 0.194 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided